Recovery · Tissue Repair · Gastrointestinal

BPC-157

Body Protection Compound-157. A synthetic 15-amino acid pentadecapeptide derived from a protective protein isolated from human gastric juice. Extensively researched for its role in angiogenesis, tendon-to-bone healing, and mucosal protection.

≥99.5% Purity HPLC MS Identity Confirmed CAS 137525-51-0 Lyophilized · GMP Synthesized

Molecular Specifications

CAS Number137525-51-0
SequenceGEPPPGKPADDAGLV
Residues15 amino acids
Mol. Weight1419.5 Da
FormulaC₆₂H₉₈N₁₆O₂₂
Half-life (SC)~4 hours
Storage−20°C (lyoph.)
Reconstituted2–8°C / 7 days
Purity
99.5%
BatchAV-2025-BPC-053

How BPC-157 Acts at the Cellular Level

BPC-157 is a pleiotropic compound with activity across multiple biological systems. Current research suggests the following primary mechanisms, based on in vitro and in vivo animal model data:

  1. VEGFR2 Upregulation → Angiogenesis

    BPC-157 significantly upregulates vascular endothelial growth factor receptor 2 (VEGFR2), driving angiogenic processes in healing tissue. This accelerates the formation of new capillary networks essential for tissue repair and oxygen delivery to ischemic areas.

  2. Nitric Oxide (NO) System Modulation

    The peptide modulates nitric oxide synthesis, demonstrating cytoprotective effects particularly in the gastrointestinal tract. This NO-dependent pathway underlies much of BPC-157's gastroprotective activity observed in gastric ulcer models.

  3. Growth Factor Receptor Upregulation in Tendon Tissue

    In tendon and ligament research models, BPC-157 upregulates growth hormone receptors (GHR) at the site of injury, enhancing GH-mediated repair signalling locally without systemic GH elevation. This contributes to accelerated tendon-to-bone healing observed in animal studies.

  4. EGF / EGFR Pathway Activation

    BPC-157 upregulates epidermal growth factor (EGF) and its receptor (EGFR), supporting mucosal regeneration in the GI tract. Studies by Sikirić et al. document significant acceleration of mucosal healing in rat models with chemically induced damage.

  5. FAK–Paxillin Pathway in Cell Migration

    In vitro research demonstrates BPC-157 activates the focal adhesion kinase (FAK)–paxillin signalling axis, accelerating fibroblast and myoblast migration toward injury sites — a critical component in organised tissue repair.

Primary references: Sikirić PC et al. (2018). "Novel cytoprotective mediator, stable gastric pentadecapeptide BPC 157." Current Pharmaceutical Design 24(18):1990–2001. DOI:10.2174/1381612824666180inclination. Chang CH et al. (2011). "The promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migration." J Appl Physiol 110(3):774–780.

Key Areas of Investigation

Tendon & Musculoskeletal Research

BPC-157 has been among the most studied synthetic peptides in orthopaedic research contexts. Animal model studies (Sprague-Dawley rats, New Zealand white rabbits) consistently demonstrate accelerated healing in Achilles tendon transection, medial collateral ligament transection, and rotator cuff tear models. Chang et al. (2011) reported statistically significant improvements in structural organisation and biomechanical tensile strength in BPC-157-treated tendons compared to controls at 8-week follow-up.

Gastrointestinal Research

The peptide's gastric origin correlates with extensive GI research. Studies from Sikirić's group at the University of Zagreb have documented protective and healing effects in models of NSAID-induced gastric ulceration, anastomotic healing, and short bowel syndrome in rodents. The cytoprotection appears to operate independently of acid suppression — a mechanistically distinct property from conventional gastroprotective agents.

Nervous System Research

Emerging preclinical research explores BPC-157's modulatory effects on the dopaminergic and serotonergic systems. Rodent studies have investigated its activity in models of depression (forced swim test, tail suspension test), with observed effects on dopamine receptor expression in the nucleus accumbens. This remains an active and early-stage area of investigation.

Inflammatory Pathway Research

BPC-157 has demonstrated significant anti-inflammatory properties in carrageenan-induced paw oedema models and in adjuvant arthritis models, apparently without classical COX-2 inhibition — suggesting an alternative mechanism potentially involving JAK-STAT or NF-κB pathway modulation. Further in vitro mechanistic work is needed to confirm the precise targets.

Research Status Note

The majority of BPC-157 research to date has been conducted in rodent models. Human clinical trial data is limited. Researchers and institutions should review the primary literature independently and apply appropriate experimental controls.

Batch Purity Documentation

Verified Purity — Independent Analysis
BPC-157 · Batch AV-2025-BPC-053
99.5%
RP-HPLC (C18) · MS/ESI+
Batch ID
AV-2025-BPC-053
Test Date
08 Mar 2025
Method
RP-HPLC C18

Certificate of Analysis — Batch AV-2025-BPC-053

Full HPLC chromatogram, mass spectrum, and identity confirmation. Issued by independent accredited laboratory. Available on request within 24 hours.

Test ParameterSpecificationResultStatus
Identity (ESI-MS)m/z consistent with MW 1419.5 Da1419.48 Da✓ Pass
Purity (RP-HPLC)≥99.0%99.5%✓ Pass
Water content (KF)≤8.0%4.2%✓ Pass
AppearanceWhite to off-white lyophilised powderConfirmed✓ Pass
Heavy metals (ICP)≤10 ppm total<2 ppm✓ Pass

Laboratory Handling Guidelines

Storage Conditions

  • Lyophilised (sealed vial): −20°C, protected from light and moisture. Stable for up to 24 months.
  • Lyophilised (opened/pierced): Use within the same research session or re-seal promptly. Store at −20°C.
  • Reconstituted solution: 2–8°C (standard laboratory refrigeration). Use within 7 days.
  • Avoid: Repeated freeze-thaw cycles. Each freeze-thaw cycle may reduce peptide integrity. Aliquot before freezing if necessary.
  • Container: Borosilicate glass preferred. Avoid excessive agitation.

Reconstitution Protocol

  • Solvent: Bacteriostatic Water (0.9% benzyl alcohol, sterile) for multi-use vials. Sterile saline for single-use protocols.
  • Volume: Add 1–2 mL per vial of 5 mg BPC-157 to achieve a concentration of 2.5–5.0 mg/mL. Adjust for required research concentration.
  • Method: Inject solvent slowly along the vial wall — not directly onto the lyophilised cake. Swirl gently until fully dissolved. Do not vortex or shake vigorously.
  • Clarity check: Reconstituted solution should be clear and colourless. Discard if cloudy, particulate, or discoloured.
  • Sterility: Use sterile technique throughout. Use 0.2 μm syringe filter if required by protocol.

Concentration Reference

1 mL BAC water + 5 mg BPC-157 = 5,000 mcg/mL (5 mg/mL). For 500 mcg/dose: 0.1 mL per administration in animal models.

For Laboratory Research Use Only. BPC-157 is a research compound not approved for human or animal therapeutic use. All handling must comply with applicable institutional biosafety and laboratory regulations. AVREA Laboratories accepts no liability for improper use.

Frequently Asked Questions

BPC-157 (Body Protection Compound-157) is a synthetic pentadecapeptide comprising 15 amino acids (sequence: GEPPPGKPADDAGLV), derived from a protective protein found in human gastric juice. CAS number 137525-51-0, molecular weight 1419.5 Da. It does not occur naturally in isolation but is derived from a fragment of the endogenous gastric protein BPC.
In rodent pharmacokinetic studies, BPC-157 has an elimination half-life of approximately 4 hours via subcutaneous route. Intragastric administration demonstrates slightly different kinetics owing to the protective environment of the gastric lumen. No human pharmacokinetic data is currently published.
BPC-157 and TB-500 (synthetic Thymosin Beta-4 fragment) are mechanistically distinct compounds. BPC-157 acts primarily via VEGFR2 and nitric oxide pathways; TB-500 acts via actin sequestration and cell motility (G-actin binding). Research often investigates them in complementary roles in tissue repair protocols, but they are separate molecules with different molecular weights and sequences.
Bacteriostatic Water (0.9% benzyl alcohol, sterile, USP-grade) is the standard solvent for research reconstitution intended for multi-use vials. Sterile isotonic saline (0.9% NaCl) may be used for single-use protocols. Acetic acid solutions are not required for BPC-157 (unlike some other peptides such as CJC-1295). Do not use plain sterile water alone — it lacks preservative action.
Reconstituted BPC-157 in bacteriostatic water is stable for approximately 7 days at 2–8°C. Beyond this window, peptide degradation may affect research accuracy. For longer-term storage post-reconstitution, aliquot and freeze at −20°C, though this introduces freeze-thaw risk. Lyophilised powder remains stable for up to 24 months at −20°C.
Yes. A full COA including RP-HPLC chromatogram, mass spectrum, and identity/purity data for batch AV-2025-BPC-053 is available on request. Contact research@avreashop.com or use the COA request button on this page. Documents are typically dispatched within 24 business hours.

Compounds Frequently Investigated Alongside BPC-157

AVREA Research Supply

BPC-157 — Batch AV-2025-BPC-053

HPLC-verified ≥99.5% purity. Available as 5 mg lyophilised vial or 500 mcg capsules. Independent COA available on request. For laboratory research use only.

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