5-amino-1-methylquinolinium small molecule — selective inhibitor of nicotinamide N-methyltransferase (NNMT). Studied for intracellular NAD⁺ elevation, SIRT1 activation, and thermogenic effects in white adipose tissue research models.
5-Amino-1MQ (5-amino-1-methylquinolinium iodide) is a small-molecule selective inhibitor of nicotinamide N-methyltransferase (NNMT). Unlike peptide research compounds, it is an orally bioavailable, room-temperature stable quinolinium derivative that crosses cell membranes readily and accumulates in tissues with high NNMT expression — notably white adipose tissue and liver.
5-Amino-1MQ is structurally a transition-state analogue of NNMT's natural substrate. It binds with high affinity (Ki low μM range) and selectivity over other methyltransferases.
With NNMT blocked, NAM is preferentially salvaged into NAD⁺ via NAMPT → NMNAT enzymatic conversion. The metabolic flux shifts from methylation/excretion toward redox cofactor regeneration.
Elevated NAD⁺ activates SIRT1 deacetylase activity, which in turn deacetylates LKB1 → activates AMPK. The combined SIRT1/AMPK axis drives mitochondrial biogenesis and metabolic efficiency.
In diet-induced obesity mouse models, 5-Amino-1MQ administration reduced fat mass by 30–40%, improved glucose tolerance, and increased white-adipose mitochondrial density. The effects appear specific to NNMT-high tissues, with skeletal muscle showing more modest changes.
Refs: Kraus D et al., Nature (2014); Neelakantan H et al., Biochem Pharmacol (2018)
NNMT expression rises with age, contributing to the well-documented age-related decline in tissue NAD⁺ levels. 5-Amino-1MQ has been studied alongside NAD⁺ precursors (NR, NMN) as a complementary approach — addressing NAD⁺ consumption rather than supplementation.
Refs: Sampath D et al., Bone (2015); Liu M et al., Cell Metab (2018)
Each batch is independently tested by a third-party ISO-accredited laboratory. The analysis below reflects Batch AV-2025-1MQ-008, tested 15 January 2025.
| Test Parameter | Method | Specification | Result | Status |
|---|---|---|---|---|
| Identity | APCI-MS / NMR (¹H, ¹³C) | Matches reference | Confirmed | PASS |
| Purity (HPLC) | RP-HPLC C18, 254nm | ≥98.0% | 99.1% | PASS |
| Residual solvents | GC-headspace | ICH Q3C limits | Below LOQ | PASS |
| Heavy metals | ICP-MS | ≤10 ppm | <2 ppm | PASS |
| Microbial limits | USP <61> | ≤100 CFU/g | Conforms | PASS |
| Form | Temperature | Duration |
|---|---|---|
| Capsules (sealed) | Room temperature, dry | 36 months |
| Bulk powder | Room temperature, desiccated | 36 months |
| Aqueous solution | 2–8°C, light-protected | ≤14 days |
No. 5-Amino-1MQ is a small organic molecule — specifically a methylated quinolinium derivative. This gives it advantages over peptide research compounds: oral bioavailability, room-temperature stability, no cold-chain requirements, and significantly longer shelf life. Like peptides, it is supplied for laboratory research use only.
NMN (nicotinamide mononucleotide) and NR (nicotinamide riboside) are NAD⁺ precursors that add to the pool by supplying substrate for synthesis. 5-Amino-1MQ takes the opposite approach: it prevents NAD⁺ depletion by blocking the methylation pathway that consumes nicotinamide. Some research protocols combine the two approaches to study additive or synergistic NAD⁺ elevation.
NNMT is highly expressed in white adipose tissue, liver, and certain cancer cell types. White adipose expression rises with obesity and age, which explains why NNMT inhibition produces more pronounced metabolic effects in these contexts. Lower expression in skeletal muscle and brain limits effects in those tissues.
Published preclinical studies have evaluated doses from 25–150 mg daily equivalent in rodent models. Higher doses do not appear to scale linearly with efficacy due to enzymatic saturation kinetics. AVREA supplies pre-dosed capsules and bulk powder; researchers should follow IACUC-approved protocols for animal dosing.
Independently verified by third-party ISO-accredited laboratory. Available as pre-dosed capsules or bulk powder.
View 5-Amino-1MQ in Store → Reconstitution Protocol Guide